• S. Afr. Med. J. · Mar 1991

    [Rapid identification methods of yeasts during fungaemia].

    • L Badenhorst, P L Botha, and M N van Rensburg.
    • Departement Mediese Mikrobiologie, Universiteit van die Oranje-Vrystaat, Bloemfontein.
    • S. Afr. Med. J. 1991 Mar 16; 79 (6): 304-6.

    AbstractA wet preparation method, modified serum test and a modified disc diffusion method were evaluated in order to identify yeasts more rapidly during fungaemia. A total of 2,932 blood cultures were processed, of which 54 (1.8%) yielded yeasts. By using these methods, Candida albicans was identified within 3 hours, after yeast growth in the blood culture was confirmed by Gram stain. The wet preparation examinations were accurate in 60% of cases and the serum test in 95% of cases. After growth was detected, yeast species other than C. albicans were identified within 24 hours using the modified disc diffusion method. C. albicans (50%), C. tropicalis (22%) and C. parapsilosis (22%) were the species most frequently isolated. Fungaemia occurred most often after antimicrobial therapy and abdominal conditions.

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