• Yufu Wang, Jing Li, Pengyu Kong, Song Zhao, Hui Yang, Chao Chen, and Jinglong Yan.
• *Department of Orthopedics Surgery, Second Hospital, Harbin Medical University, Harbin, P.R. China †Department of Pathology and Center of Electron Microscope, School of Basic Medicine, Harbin Medical University, Harbin, P.R. China; and ‡Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Canada.
• Spine. 2015 Jan 15;40(2):95-101.

Study DesignVaccination of spinal cord injury (SCI) mice with myelin basic protein-derived peptide (A91) pulsed dendritic cells (DC) to enhance brain-derived neurotrophic factor and neurotrophin-3 (NT-3) expression in injured spinal cord.ObjectiveTo investigate the effect of A91-pulsed DC (A91-DC) on expression of neurotrophic factor in injured spinal cord.Summary Of Background DataSCI leads to progressive secondary tissue degeneration, and no satisfactory treatment is currently available. Accumulating evidence indicates that administration of neurotrophic factors to injured spinal cord is partially successful at promoting nerve tissue repair. However, most of strategy can cause secondary injury and limiting their wide clinical application.MethodsProliferation of T cells and the capability of CD4 T cells to secret neurotrophic factors were first measured in vitro to demonstrate the stimulus action of the A91-DC. In SCI mice model, enzyme-linked immunosorbent assay and immunofluorescence was employed to investigate the brain-derived neurotrophic factor and NT-3 expression in injured spinal cord. Furthermore, the neuroprotective effect of A91-DC in injured spinal cord was examined through histology measurement.ResultsIn this study, we demonstrated that A91-DC promoted the capability of T cells to secret neurotrophic factors and in the subacute phase of SCI. Moreover, vaccination with A91-DC enhanced the expression level of brain-derived neurotrophic factor and NT-3 and exerted neuroprotective effect in injured spinal cord.ConclusionThe findings of study demonstrate that the therapeutic strategy of vaccination A91-DC is a potential minimally invasive approach that could provide strong neurotrophic factor support after SCI.

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