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Journal of neurovirology · Dec 2007
Anterograde transsynaptic tracing in the murine somatosensory system using Pseudorabies virus (PrV): a "live-cell"-tracing tool for analysis of identified neurons in vitro.
- Markus Rothermel, Nicole Schöbel, Nils Damann, Barbara G Klupp, Thomas C Mettenleiter, Hanns Hatt, and Christian H Wetzel.
- Lehrstuhl für Zellphysiologie, Ruhr-Universität, Bochum, Germany. Markus.Rothermel@rub.de
- J. Neurovirol. 2007 Dec 1; 13 (6): 579-85.
AbstractThe Pseudorabies virus (PrV) strain Bartha is widely used as a tool for retrograde transneuronal tracing in mammals. Traced neurons can be identified in cell culture allowing the analysis of their physiological features ("live-cell"-tracing). Compared to PrV-Bartha, PrV-Kaplan is known for higher virulence and transsynaptic spread in both retrograde and anterograde direction. Herein we assess the authors assess PrV-Kaplan for transsynaptic anterograde "live-cell"-tracing. Following intranasal application in mice, labelled trigeminal and brainstem neurons could be identified in vitro. Detailed electrophysiological analysis indicated that viral infection did not affect neuronal properties, making PrV-Kaplan eligible for functional analysis of identified neurons within somatosensory systems.
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