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Am. J. Trop. Med. Hyg. · Dec 2020
A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2.
- Yee Ling Lau, Ilyiana Binti Ismail, Nur Izati Binti Mustapa, Meng Yee Lai, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M Peariasamy, Yee Leng Lee, and Pik Pin Goh.
- Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
- Am. J. Trop. Med. Hyg. 2020 Dec 1; 103 (6): 2350-2352.
AbstractA simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.
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