• Gregory M T Hare, Elaine Liu, Andrew J Baker, and C David Mazer.
• Departments of Anesthesia and Critical Care, Cara Phelan Centre for Trauma Research, The Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael's Hospital, University of Toronto, Toronto, ON, M5B 1W8, Canada.
• Intensive Care Med. 2009 Nov 1; 35 (11): 1925-33.

PurposeTo determine the oxygen affinity for optimal tissue oxygen delivery with a hemoglobin-hydroxyethyl starch conjugate (HRC 101).MethodsAnesthetized rats were hemodiluted (180 ml kg(-1)) with low (P(50) approximately 70 mmHg) or high affinity (P(50) approximately 14 mmHg) HRC 101 at hemoglobin (Hb) concentrations near 100 or 70 g l(-1) (n = 6-8). Hippocampal tissue oxygen tension (P(Br)O(2)), blood flow, arterial blood gases, Hb, hematocrit (Hct) and lactate were measured. Data (mean +/- SD) were analyzed by two-way ANOVA.ResultsHemodilution reduced the hematocrit to 1 +/- 1% in all groups. P(Br)O(2) was best maintained after hemodilution with low affinity HRC 101 at Hb 100 and 70 g l(-1) (25.2 +/- 7.6 and 16.6 +/- 8.3 torr, respectively). P(Br)O(2) decreased (9.5 +/- 9.3 torr, P < 0.05) and serum lactate levels increased (5.0 +/- 1.7 mmol l(-1), P < 0.05) following hemodilution with the high affinity HRC 101 (Hb 100 g l(-1)).ConclusionsHRC 101 with a lower oxygen affinity favored tissue perfusion and maintained P(Br)O(2) after near complete blood volume exchange in rats.

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