• Yu-Li Chang, Tian-Sheng Sun, Xiao-Dong Zhang, Zhou-Yong Ma, Qiang Su, Da-Jiang Ren, Li-Ren Zhang, and Shao-Ting Xu.
• Department of Orthopaedics, Beijing Army General Hospital, Beijing 100700, China.
• Zhonghua Yi Xue Za Zhi. 2008 Jun 17; 88 (23): 1643-7.

ObjectivesTo investigate in vive osteogenic potential in size-critical bone defect after percutaneous autologous grafting of culture-expanded rabbit autologous BMSCs, osteo-induced BMSCs and combination of both.MethodsBMSCs were cultured and then induced with osteogenic supplement (OS) medium. BMSCs with and without OS induction were collected and percutaneously autologously injected respectively into the 15 mm bone defect of 20 experimental rabbit model. The grafts were BMSCs, osteo-induced BMSCs, BMSCs and osteo-induced BMSCs, BMP combined with fibrin sealant, and 0.9% NaCl solution. Osteogenesis at the defect areas were observed by regular radiography, histology and biomechanics.ResultsThe group transplanted with BMSCs + osteo-induced BMSCs achieved complete bone healing with medullary cavity united, which showed the largest quantity of new bone measured by X-ray analysis, and also their maximal load were better than those in other groups.ConclusionThe bone-forming ability of rabbit osteo-induced BMSCs combined with BMSCs in bone defect is superior to those of BMSCs and osteo-induced BMSCs.

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