• Acta Ophthalmol Scand · Oct 1997

    Comparative Study

    Comparative study on the effects of different growth factors on migration of bovine corneal endothelial cells during wound healing.

    • A Thalmann-Goetsch, K Engelmann, and J Bednarz.
    • Universitäts-Augenklinik, Hamburg, Germany.
    • Acta Ophthalmol Scand. 1997 Oct 1; 75 (5): 490-5.

    PurposeIn a comparative study we investigated the effects of epidermal growth factor, acidic and basic fibroblast growth factor, transforming growth factor-beta1, and vascular endothelial growth factor on proliferation and migration of cultured bovine corneal endothelial cells during wound healing.MethodsCell proliferation was determined by incorporation of 5-bromo-2'-deoxy-uridine and by cell counting. To investigate cell migration, we established a method to produce identically shaped cell-free areas in monolayers of cultured cells and documented the wound-healing process.ResultsConcerning the five tested growth factors, only epidermal growth factor as well as basic and acidic fibroblast growth factor stimulated DNA synthesis, cell proliferation, and migration during wound healing. Vascular endothelial growth factor stimulated migration during wound healing without influencing DNA synthesis or cell proliferation.ConclusionWhereas epidermal growth factor and the basic and acidic forms of fibroblast growth factor enhance proliferation as well as migration during wound healing, vascular endothelial growth factor seems to be a stimulatory agent specific for corneal endothelial cell migration.

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