• Marina Gomes Galvani, Moreira AntunesHanna KarenHKDepartment of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil., Marcos Monico-Neto, Veronica Quispe Yujra, Carla Maximo Prado, Hananiah Tardivo Quintana, Flavia de Oliveira, and Daniel Araki Ribeiro.
• Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil.
• Med Princ Pract. 2021 Jan 1; 30 (3): 253261253-261.

ObjectivesThe aim of the study was to study the Janus kinase/tyrosine kinase-activated transduction factor (JAK/STAT) signaling pathway and myogenesis on the masseter muscle after sleep deprivation and to investigate the role of stress in this scenario.Subjects And MethodsA total of 18 male Wistar rats were divided into the following groups: control (n = 6): animals were not submitted to any procedures, and paradoxical sleep deprivation and vehicle (PSD + V; n = 6): animals were subjected to PSD for 96 h and (PSD + MET; n = 6): animals were subjected to PSD for 96 h with administration of metyrapone. Paradoxical sleep deprivation was performed by the modified multiple platforms method. Histopathological analysis, histomorphometry, and immunohistochemistry were performed.ResultsThe results showed the presence of inflammatory infiltrate in the PSD + V and PSD + MET groups and atrophy. Histomorphometry showed that the cellular profile area decreased, while cellular density increased in both experimental groups. Expression of p-STAT 3, MyoD, and MyoG increased in the PSD + V group, while the PSD + MET group showed increased expression of IL-6 and p-STAT 3.ConclusionOur results suggest that sleep deprivation induces an inflammatory response and atrophy in the masseter muscle of rats.© 2021 The Author(s) Published by S. Karger AG, Basel.

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