• Lucía Melguizo-Rodríguez, Francisco Javier Manzano-Moreno, Elvira De Luna-Bertos, Ana Rivas, Javier Ramos-Torrecillas, Concepción Ruiz, and Olga García-Martínez.
• Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain.
• Eur. J. Clin. Invest. 2018 Apr 1; 48 (4).

BackgroundOsteoporosis is a skeletal disorder characterized by compromised bone strength that predisposes individuals to an increased risk of fracture. Previous in vivo and in vitro studies have reported that phenolic compounds present in extra virgin olive oil have a beneficial effect on osteoblasts in terms of increase cell proliferation. The aim of this study was to determine whether phenolic compounds present in olive oil could modify the expression of cell differentiation markers on osteoblasts.Study DesignAn in vitro experimental design was performed using MG-63 osteoblasts cell line.MethodsMG63 cells were exposed to different doses of luteolin, apigenin, or p-coumaric, caffeic or ferulic acid. Alkaline phosphatase (ALP) was evaluated by spectrophotometry and antigen expression (cluster of differentiation [CD] 54, CD80, CD86 and HLA-DR) by flow cytometry.ResultsAt 24 hour, treated groups showed an increased ALP and modulated antigen profile, with respect to the nontreated group.ConclusionThese results demonstrate that the phenolic compounds studied induce cell maturation in vitro, increasing ALP synthesis and reducing the expression of antigens involved in immune functions of the osteoblast which would improve bone density.© 2018 Stichting European Society for Clinical Investigation Journal Foundation.

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