• Eur Spine J · Aug 2013

    Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow.

    • Marc Soubeyrand, Elisabeth Laemmel, Charles Court, Arnaud Dubory, Eric Vicaut, and Jacques Duranteau.
    • Equipe universitaire 3509 Paris VII-Paris XI-Paris XIII, Microcirculation, Bioénergétique, Inflammation et Insuffisance circulatoire aiguë, Paris Diderot-Paris VII University, Paris, France. soubeyrand.marc@wanadoo.fr
    • Eur Spine J. 2013 Aug 1;22(8):1810-9.

    PurposesCerebrospinal fluid (CSF) pressure elevation may worsen spinal cord ischaemia after spinal cord injury (SCI). We developed a rat model to investigate relationships between CSF pressure and spinal cord blood flow (SCBF).MethodsMale Wistar rats had SCI induced at Th10 (n = 7) or a sham operation (n = 10). SCBF was measured using laser-Doppler and CSF pressure via a sacral catheter. Dural integrity was assessed using subdural methylene-blue injection (n = 5) and myelography (n = 5).ResultsThe SCI group had significantly lower SCBF (p < 0.0001) and higher CSF pressure (p < 0.0001) values compared to the sham-operated group. Sixty minutes after SCI or sham operation, CSF pressure was 8.6 ± 0.4 mmHg in the SCI group versus 5.5 ± 0.5 mmHg in the sham-operated group. No dural tears were found after SCI.ConclusionOur rat model allows SCBF and CSF pressure measurements after induced SCI. After SCI, CSF pressure significantly increases.

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