• Laboratory animals · Oct 2014

    Access to cerebrospinal fluid in piglets via the cisterna magna: optimization and description of the technique.

    • Noemi Romagnoli, Domenico Ventrella, Massimo Giunti, Francesco Dondi, Nicolina C Sorrentino, Alessandro Fraldi, Enrico M Surace, and Maria L Bacci.
    • Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy noemi.romagnoli@unibo.it.
    • Lab. Anim. 2014 Oct 1; 48 (4): 345-8.

    AbstractThe collection of cerebrospinal fluid is necessary in order to determine its composition. It can then be used to diagnose various diseases. The aim of the study was to develop and optimize a technique for performing safe centesis for the collection of cerebrospinal fluid in piglets and its injection through the cisterna magna. The study was divided into three phases: (1) anatomical study of cadavers, (2) in vivo application of the technique and (3) observation of recovery time. The proposed technique resulted in a safe puncture of the cisterna magna. The authors identified and confirmed the correspondence of the crista occipitalis and the wings of the atlas with the external landmarks on the cadaver by means of direct radiological visualization. The punctures were performed successfully at the first attempt in 11 out of 12 anaesthetized piglets. The technique herein described provides a reproducible safe and easy route for approaching the cisterna magna for cerebrospinal fluid collection, drug administration and gene delivery.© The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

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