• Muhammed Selcuk Ozer, Huseyin Alperen Yildiz, Canet Incir, DegerMuslim DoganMDDepartment of Urology, Edirne Sultan 1. Murat State Hospital, Edirne, Turkey., Ozan Bozkurt, Gul Ergor, Yesim Tuncok, Nuran Esen, and Ahmet Adil Esen.
• Department of Urology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
• Int. J. Clin. Pract. 2021 Jul 1; 75 (7): e14193.

ObjectiveThe aim of this study is to establish the optimal non-invasive urine sample collection method for the microbiota studies.MethodologyTwelve men with bladder carcinoma underwent first voided and midstream urine collection. Urine samples were analysed using V3-V4 regions of bacterial 16s ribosomal RNAs. Bacterial groups with relative abundance above 1% were analysed in first voided urine and midstream urine samples at phylum, class, order and family level. At the genus level, all of the identified bacterial groups' relative abundances were analysed. The statistical significance (P < .05) of differences between first voided and midstream urine sample microbiota was evaluated using the Wilcoxon test.ResultsAccording to the analysis, 8 phyla, 14 class, 23 orders, 39 families and 29 different genera were identified in the first voided and the midstream urine samples. Statistical differences were not identified between first voided and midstream urine samples of all bacteria groups except the Clostridiales at order level (p:0.04) and Clostridia at class level (P: .04).ConclusionsEither first voided or midstream urine samples can be used in urinary microbiota studies as we determined that there is no statistically significant difference between them regarding the results of 16s ribosomal RNA analysis.© 2021 John Wiley & Sons Ltd.

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