• Ligia Petrica, Elena Hogea, Florica Gadalean, Adrian Vlad, Mihaela Vlad, Victor Dumitrascu, Silvia Velciov, Cristina Gluhovschi, Flaviu Bob, Sorin Ursoniu, Dragos Catalin Jianu, Petru Matusz, Agneta-Maria Pusztai, Andrei Motoc, Octavian M Cretu, Dana Radu, Oana Milas, Alina Golea-Secara, Anca Simulescu, Maria Mogos-Stefan, Mihaela Patruica, Lavinia Balint, Silvia Ienciu, Daliborca Vlad, and Roxana Popescu.
• Dept. of Internal Medicine II - Division of Nephrology, "Victor Babes" University of Medicine and Pharmacy Timisoara, Romania, Eftimie Murgu Sq. no. 2, 300041 Timisoara, RO; County Emergency Hospital Timisoara, RO.
• Int J Med Sci. 2021 Jan 1; 18 (10): 209321012093-2101.

AbstractAims: Long noncoding RNAs (lncRNAs) play key roles in the pathophysiology of DKD involving actions of microRNAs (miRNAs). The aims of the study were to establish the involvement of selected lncRNAs in the epigenetic mechanisms of podocyte damage and tubular injury in DKD of type 2 diabetes mellitus (DM) patients in relation to a particular miRNAs profile. Methods: A total of 136 patients with type 2 DM and 25 healthy subjects were assessed in a cross-sectional study concerning urinary albumin: creatinine ratio (UACR), eGFR, biomarkers of podocyte damage (synaptopodin, podocalyxin) and of proximal tubule (PT) dysfunction (Kidney injury molecule-1-KIM-1, N-acetyl-D-glucosaminidase-NAG), urinary lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), nuclear-enriched abundant transcript 1 (NEAT1), myocardial infarction-associated transcript (MIAT), taurine-upregulated gene 1 (TUG1), urinary miRNA21, 124, 93, 29a. Results: Multivariable regression analysis showed that urinary lncMALAT1 correlated directly with urinary synaptopodin, podocalyxin, KIM-1, NAG, miRNA21, 124, UACR, and negatively with eGFR, miRNA93, 29a (p<0.0001; R2=0.727); urinary lncNEAT1 correlated directly with synaptopodin, KIM-1, NAG, miRNA21, 124, and negatively with eGFR, miRNA93, 29a (p<0.0001; R2=0.702); urinary lncMIAT correlated directly with miRNA93 and 29a, eGFR (p<0.0001; R2=0.671) and negatively with synaptopodin, KIM-1, NAG, UACR, miRNA21, 124 (p<0.0001; R2=0.654); urinary lncTUG1 correlated directly with eGFR, miRNA93, 29a, and negatively with synaptopodin, podocalyxin, NAG, miRNA21, 124 (p<0.0001; R2=0.748). Conclusions: In patients with type 2 DM lncRNAs exert either deleterious or protective functions within glomeruli and PT. LncRNAs may contribute to DKD through modulating miRNAs expression and activities. This observation holds true independently of albuminuria and DKD stage.© The author(s).

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