• Zhongguo Wei Zhong Bing Ji Jiu Yi Xue · Jun 2005

    [Effects of combined naloxone and methylprednisolone on nuclear factor-kappaB expression in the lung in acute lung injury in rats].

    • Qiu-jin Zhang, Hong Shen, Wei Zhang, Yin-ping Li, and Tan-shi Li.
    • Emergency Department, General Hospital of PLA, Beijing 100853, China.
    • Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2005 Jun 1; 17 (6): 370-2.

    ObjectiveTo investigate the effects of a combination of naloxone and methylprednisolone on nuclear factor-kappaB (NF-kappaB) p65 expression in the lung tissue in lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats.MethodsALI models were reproduced by intratracheal instillation of LPS (3 mg/kg). Four hours after LPS instillation, rats were randomly divided into five groups: normal saline group, LPS group, methylprednisolone group, naloxone group (LPS+naloxone) and combined drug group (LPS+naloxone+methylprednisolone). The level of interleukin-8 (IL-8) in serum was measured by immunoassay. Meanwhile, the expression of NF-kappaB p65 in the lung tissue was determined with immunohistochemical staining.ResultsNaloxone and methylprednisolone significantly reduced the LPS-induced increase in IL-8 concentrations in serum in vivo, and suppressed the activation of NF-kappaB p65 in the lung tissue.ConclusionNF-kappaB activation is involved in the LPS-induced ALI in rats. Combination of naloxone and methylprednisolone could suppress the increase of IL-8 content and NF-kappaB activation in the lung tissue of rat in vivo in our experiment.

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