• Barbara Pregl Breznik, Borut Kovačič, and Veljko Vlaisavljević.
• Department of Reproductive Medicine and Gynaecologic Endocrinology, University Medical Centre, Maribor, Slovenia. breznik.barbara@siol.net
• Fertil. Steril. 2013 Apr 1; 99 (5): 1233-41.

ObjectiveTo determine the diagnostic value of the following sperm function tests in predicting the fertilizing ability of spermatozoa in conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI): hyaluronan-binding assay (HBA), DNA fragmentation (Halosperm), and hyperactivity.DesignProspective study.SettingUniversity medical center.Patient(S)133 couples undergoing infertility treatment with IVF/ICSI.Intervention(S)Analysis of sperm DNA fragmentation, hyaluronan-binding ability, and hyperactivation on washed semen samples used for the insemination of oocytes.Main Outcome Measure(S)Correlation between the results of sperm function tests and the fertilization rate (FR) or embryo quality (EQ) after IVF and ICSI. Comparison of the sperm DNA fragmentation, hyperactivation, and hyaluronan binding ability between cycles with less than 50% (group 1) and more than 50% (group 2) of oocytes fertilized after IVF.Result(S)Both FR and EQ in IVF cycles negatively correlated with sperm DNA fragmentation. Furthermore, a positive correlation was observed between FR and hyaluronan-binding ability or induced hyperactivity. The semen samples from the IVF cycles with low FR (group 1) were characterized by statistically significantly higher sperm DNA fragmentation and lower hyaluronan-binding ability in comparison with semen samples from the group with high levels of fertilization (group 2). In ICSI cycles, no relationship was found between sperm function tests and FR or EQ.Conclusion(S)The Halosperm test, the HBA test, and induced hyperactivity are useful in predicting the ability of spermatozoa to fertilize oocytes in IVF and are helpful in distinguishing semen samples suitable for IVF or ICSI.Copyright © 2013 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

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