• Dan Li, Ya-Na Ren, Jie Yang, Yi-Ming Yang, Chun-Yan Li, Ru-Feng Xie, and Hua-Hua Fan.
• Huashan Hospital of Fudan University united by Blood Center, Shanghai 200040, China.
• Zhonghua Xue Ye Xue Za Zhi. 2011 Jun 1; 32 (6): 404-7.

ObjectiveTo study the influence of human plasma exosomes-like vesicles on the regulatory function of macrophages.MethodsThe exosomes-like vesicles were purified from healthy donors plasma with a series of high-speed centrifugation and ultrafiltration. Macrophages were derived from cultured human blood monocytes. The molecular markers of macrophages were assayed by FACS. After cultured with exosomes-like vesicles, the changes of macrophages cytoplasma Ca(2+), and related genes and proteins were assayed by FACS, RT-PCR and Western Blot, respectively.ResultsAfter cultured with exosomes-like vesicles, mean fluorescent intensity (MFI) of macrophages cytoplasma Ca(2+) was increased. The vesicles enhanced macrophages to express cytokines genes, the expression of IL-1β and TNF-α genes being increased by 0.85 and 1.69 times respectively at 2 h, and that of IL-6 gene 3.7 times compared with the control at 8 h. However, the vesicles inhibited the expression of macrophages IL-10 gene, had no influence on the Frizzled5 receptor expression and could induce CaMKII phosphorylation.ConclusionsExosomes-like vesicles can up-regulat macrophages expression of inflammatory cytokines genes, and increase the secretion of inflammatory cytokines by activating the Wnt5A-Ca(2+) signaling pathway.

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