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Respiration physiology · Jun 2001
Comparative StudyRegulation of secretion from mucous and serous cells in the excised ferret trachea.
- C Kishioka, K Okamoto, J Kim, and B K Rubin.
- Department of Otolaryngology, Mie University School of Medicine, Tsu, Japan.
- Respir Physiol. 2001 Jun 1; 126 (2): 163-71.
AbstractMucus hypersecretion is an important characteristic of many airway diseases. Mucin is the major component of mucus, and is secreted from surface goblet cells of the airway epithelium and mucous cells of submucosal glands. Lysozyme is an enzyme secreted by serous cells of airway submucosal glands. We hypothesized that secretagogues acting through different pathways would have different effects on tracheal mucin and lysozyme secretion. We used a sandwich enzyme-linked lectin assay (ELLA) to measure mucin-like glycoprotein secretion and a spectrophotometric method to measure lysozyme secretion from isolated ferret tracheal segments. We evaluated the secretory response to four secretagogues; prostaglandin F(2alpha) (PGF(2alpha)), adenosine triphosphate (ATP), methacholine (MCh), and human neutrophil elastase (HNE). Each agent stimulated mucin and lysozyme secretion. The relative potency was PGF(2alpha)< or =ATP
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