• Zichen Song, Leilei Meng, Zhixiang He, Jing Huang, Fang Li, Jingjing Feng, Zhuoran Jia, Yue Huang, Wei Liu, Anding Liu, and Haoshu Fang.
• Department of Pathophysiology, Anhui Medical University, Hefei, China.
• Shock. 2021 Dec 1; 56 (6): 106610791066-1079.

ObjectivesTo explore the role of LPS binding protein (LBP) in metabolism and optimize sepsis treatment.DesignA sepsis model was established by injecting LPS into LBP-/- rats and WT rats and observing changes in the liver over time (0, 1, 6, and 24 h).SettingDetecting liver inflammation and injury. Optimizing the treatment of sepsis.SubjectsWT rats and LBP-/- rats.InterventionsWe established a sepsis model by injecting LPS intravenously.Measurements And Main ResultsFirst, we induced sepsis in WT and LBP-/- rats with LPS. The rats were sacrificed, and serum and liver samples were collected at 1, 6, and 24 h after LPS injection. We found that the deletion of LBP reduced LPS-induced liver inflammation and injury at 1 and 6 h. Ballooning degeneration was clearly present in LBP-/- rat livers at 24 h after LPS injection. We found that mitochondrial damage and reactive oxygen species (ROS) levels were higher in LBP-/- rat livers than in WT rat livers at 24 h after LPS injection. According to the transcriptomic results, the peroxisome proliferator-activated receptor (PPAR) pathway may be the reason for lesions in LBP-/- rats. To further investigate the function of PPARα in sepsis, we inhibited mTOR with rapamycin and examined mitochondrial injury and ROS levels. The levels of mitochondrial damage and ROS were reduced after LBP-/- rats were pretreated with rapamycin in the context of LPS-induced sepsis. Inhibiting CYP4a2, one of the PPARα-target gene products, reduced the level of LPS-induced ROS in LBP-/- rats.ConclusionLBP protects hepatic mitochondria against LPS-induced damage via the LBP-PPARα-CYP4a2 signaling pathway.Copyright © 2021 by the Shock Society.

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