Development of low-cost inverted microscope to detect early

Plos One · Mar 2010

Development of low-cost inverted microscope to detect early growth of Mycobacterium tuberculosis in MODS culture.

The microscopic observation drug susceptibility (MODS) assay for rapid, low-cost detection of tuberculosis and multidrug resistant tuberculosis depends upon visualization of the characteristic cording colonies of Mycobacterium tuberculosis in liquid media. This has conventionally required an inverted light microscope in order to inspect the MODS culture plates from below. Few tuberculosis laboratories have this item and the capital cost of $5,000 for a high-end microscope could be a significant obstacle to MODS roll-out. ⋯ In this work we describe the development of a low-cost artesenal inverted microscope that can operate in both a standard or digital mode to effectively replace the expensive commercial inverted light microscope, and an integrated system that could permit a local and remote diagnosis of tuberculosis.

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- Mirko Zimic, Abner Velazco, Germán Comina, Jorge Coronel, Patricia Fuentes, Carmen G Luna, Patricia Sheen, Robert H Gilman, and David A J Moore.
- Unidad de Bioinformática y Laboratorio de Enfermedades Infecciosas, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, San Martín de Porras, Lima, Perú
- Plos One. 2010 Mar 23; 5 (3): e9577.
BackgroundThe microscopic observation drug susceptibility (MODS) assay for rapid, low-cost detection of tuberculosis and multidrug resistant tuberculosis depends upon visualization of the characteristic cording colonies of Mycobacterium tuberculosis in liquid media. This has conventionally required an inverted light microscope in order to inspect the MODS culture plates from below. Few tuberculosis laboratories have this item and the capital cost of $5,000 for a high-end microscope could be a significant obstacle to MODS roll-out.MethodologyWe hypothesized that the precise definition provided by costly high-specification inverted light microscopes might not be necessary for pattern recognition.SignificanceIn this work we describe the development of a low-cost artesenal inverted microscope that can operate in both a standard or digital mode to effectively replace the expensive commercial inverted light microscope, and an integrated system that could permit a local and remote diagnosis of tuberculosis.

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Development of low-cost inverted microscope to detect early growth of Mycobacterium tuberculosis in MODS culture.

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