• Biomed. Pharmacother. · Jul 2018

    lncRNA NEAT1 competes against let-7a to contribute to non-small cell lung cancer proliferation and metastasis.

    • Li Qi, Fang Liu, Feng Zhang, Shuai Zhang, LiYan Lv, Yi Bi, and Yan Yu.
    • Department of Radiation Oncology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
    • Biomed. Pharmacother. 2018 Jul 1; 103: 1507-1515.

    AbstractNon-small cell lung cancer (NSCLC) accounts for >80% of diagnosed cases of lung cancer worldwide. Although multiple genes are altered in NSCLC, the precise mechanism of NSCLC requires further investigation. Nuclear paraspeckle assembly transcript (NEAT)1 is one of the long non-coding RNAs implicated in multiple types of cancer regulation. However, the role of NEAT1 in NSCLC is poorly understood. In this study, we investigated the function of NEAT1 in NSCLC and its related molecular mechanism. We demonstrated via real-time polymerase chain reaction that NEAT1 was significantly increased in NSCLC tissues and cell lines, suggesting a potential role of NEAT1 in NSCLC. CCK-8 assay and colony-formation assays showed that silencing of NEAT1 by siRNA pool inhibited NSCLC cell proliferation. An increased population of apoptotic cells was detected upon NEAT1 depletion by acridine orange/ethidium bromide staining compared to control cells. A549 cells depleted of NEAT1 exhibited reduced invasion and migration, characterized by wound healing assay and Transwell assay. To uncover the molecular mechanism of NEAT1 in lung cancer, we identified that NEAT1 could be a competing endogenous RNA against let-7a, and insulin-like growth factor (IGF)-2 could be a direct target of let-7a. These were characterized by reciprocal expression between NEAT1/IGF-2 and let-7a. Luciferase reporter assay validated direct binding of NEAT1/let-7a and let-7a/IGF-2. Exogenous IGF-2 expression reversed NEAT1-knockdown-induced growth inhibition assessed by CCK-8 assay and colony-formation assay. In conclusion, NEAT1 regulates lung cancer cell progression by competing endogenous RNA network of NEAT1/let-7a/IGF2. Our findings provide a novel insight into the biological function of NEAT1 in lung cancer and NEAT1 could be a potential therapeutic target for lung cancer.Copyright © 2018 Elsevier Masson SAS. All rights reserved.

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