• Xiaoping Zhou, Jim Ren, Erika Brown, David Schneider, Yessi Caraballo-Lopez, and James J Galligan.
• Department of Pharmacology and Toxicology, Neuroscience Program, Life Science B400, Michigan State University, East Lansing, MI 48824, USA.
• J. Pharmacol. Exp. Ther. 2002 Sep 1; 302 (3): 889-97.

AbstractThe electrophysiological and pharmacological properties of nicotinic acetylcholine receptors (nAChRs) were studied in guinea pig small intestinal myenteric neurons maintained in culture or in acutely isolated preparations. Acetylcholine and nicotine caused inward currents that desensitized in approximately 4 s. The current-voltage (I-V) relationship rectified inwardly with a reversal potential near 0 mV. The agonist rank order potency was 1,1-dimethyl-4-phenyl-piperazinium > acetylcholine = nicotine > cytisine. Agonist-induced currents were blocked by nAChR antagonists with a rank order potency of mecamylamine > hexamethonium > dihydro-beta-erythroidine (DHbetaE); mecamylamine and DHbetaE exhibit high potency at beta4 and beta2 subunit-containing nAChRs, respectively. alpha-Bungarotoxin (0.1 microM) or alpha-methyllycaconitine (0.1 microM), antagonists that block nAChRs containing alpha7 subunits, did not affect acetylcholine-induced responses. Immunohistochemical studies revealed that nearly every neuron in culture was labeled by an antibody (mAb35) that recognizes nAChR alpha3 and alpha5 subunits. Antibodies selective for alpha3, alpha5, or beta2 subunits also stained most neurons, whereas an alpha7 subunit antibody revealed very few neurons. In neurons in the intact myenteric plexus from newborn and adult guinea pigs, local application of acetylcholine (1 mM) and cytisine (1 mM) caused similar amplitude depolarizations, and these responses were blocked by nAChR antagonists with a rank order potency of mecamylamine > hexamethonium > DHbetaE. These data indicate that myenteric neurons maintained in culture predominantly express nAChRs composed of alpha3, alpha5, beta2, and beta4 subunits. These subunits may be in a homogeneous population of receptors with unique pharmacological properties, or multiple receptors of different subunit composition may be expressed by individual neurons.

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