• Rev. Inst. Med. Trop. Sao Paulo · Sep 1998

    Case Reports

    PCR-based diagnosis of a case of herpetic whitlow in an AIDS patient.

    • M L Nogueira, A F Oliveira, J G Araujo, M A Gallo, J G Fonseca, C A Bonjardim, P C Ferreira, and E G Kroon.
    • Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
    • Rev. Inst. Med. Trop. Sao Paulo. 1998 Sep 1; 40 (5): 317-9.

    AbstractHerpetic infections are common complications in AIDS patients. The clinical features could be uncommon and antiviral chemotherapy is imperative. A rapid diagnosis could prevent incorrect approaches and treatment. The polymerase chain reaction is a rapid, specific and sensible method for DNA amplification and diagnosis of infectious diseases, especially viral diseases. This approach has some advantages compared with conventional diagnostic procedures. Recently we have reported a new PCR protocol to rapid diagnosis of herpetic infections with suppression of the DNA extraction step. In this paper we present a case of herpetic whitlow with rapid diagnosis by HSV-1 specific polymerase chain reaction using the referred protocol.

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