• Diagnostics (Basel) · May 2020

    Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis.

    • Chao-Ju Chen, Li-Ling Hsieh, Shu-Kai Lin, Chu-Feng Wang, Yi-Hui Huang, Shang-Yi Lin, and Po-Liang Lu.
    • Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan.
    • Diagnostics (Basel). 2020 May 21; 10 (5).

    AbstractCoronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs.

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