• Turk J Med Sci · Oct 2021

    History repeats itself: Horse originated Hyperimmune sera production against SARS CoV-2.

    • Ahu Pakdemirli, Elvin Çalışkan, Sabri Hacıoğlu, Erdem Danyer, Özlem Kardoğan, KurtZüleyha ErgünZEVeterinary Control Central Research Institute, Ankara, Turkey., Özcan Yıldırım, Hakan Taşkaya, Bora Ündar, Yasemin Sezgin, Gencay Ergin, Himmet Ekici, Ufuk Ülker, Erkan Taçbaş, Şahin Çakır, Ramazan Bülbül, Mustafa Bebek, Meral Sarper, Dilek Dülger, Ümmü Sena Sarı, Fatma Ergin, Özden Kabaklı, and Cevdet Yaralı.
    • Department of Physiology, Gülhane School of Medicine, University of Health Sciences, Ankara, Turkey.
    • Turk J Med Sci. 2021 Oct 21; 51 (5): 226322732263-2273.

    Background/Aim/AimSARS-CoV-2 disease was announced as a pandemic by The World Health Organization in early 2020. It is still threatening the world population. Here, we aimed to produce hyperimmune sera that contain immunoglobulin G and F(ab')2 fragments sourced from horse antibodies as an urgent response to the pandemic.Materials And MethodsSARS-CoV-2 was produced and inactivated with three different methods [formaldehyde (FA), formaldehyde, and binary ethylene amine (FA + BEI), and heat treatment]. After in vitro inactivation control, immunogens were mixed with Freund’s adjuvant, thereafter horses (n: 2 for FA, 4 for FA + BEI, 2 for heat inactivation) and New Zealand rabbits (n: 6 for FA, 6 fo r FA + BEI, 6 for heat inactivation) were immunized four times. Neutralizing antibody levels of the sera were measured at the 4th, 6th, and 8th weeks. When the antibodies were detected at the peak level, plasma was collected from horses and hyperimmune sera procured after the purification process.ResultsHorses and rabbits produced highly neutralizing antibodies against the SARS-CoV-2 in FA and FA + BEI inactivation groups, foreign proteins were removed effectively after purification.ConclusionThis study presents a profitable practice to develop specific antisera in horses against SARS-CoV-2 for emergency and low-cost response. In further studies, new purification methods can be used to increase the efficiency of the final product.This work is licensed under a Creative Commons Attribution 4.0 International License.

      Pubmed     Free full text   Copy Citation     Plaintext  

      Add institutional full text...

    Notes

     
    Knowledge, pearl, summary or comment to share?
    300 characters remaining
    help        
    You can also include formatting, links, images and footnotes in your notes
    • Simple formatting can be added to notes, such as *italics*, _underline_ or **bold**.
    • Superscript can be denoted by <sup>text</sup> and subscript <sub>text</sub>.
    • Numbered or bulleted lists can be created using either numbered lines 1. 2. 3., hyphens - or asterisks *.
    • Links can be included with: [my link to pubmed](http://pubmed.com)
    • Images can be included with: ![alt text](https://bestmedicaljournal.com/study_graph.jpg "Image Title Text")
    • For footnotes use [^1](This is a footnote.) inline.
    • Or use an inline reference [^1] to refer to a longer footnote elseweher in the document [^1]: This is a long footnote..

    hide…