• Daniel Alberto Girón-Pérez, Rocío Alejandra Ruiz-Manzano, Alma Betsaida Benitez-Trinidad, Guadalupe Herminia Ventura-Ramón, Carlos Eduardo Covantes-Rosales, Ansonny Jhovanny Ojeda-Durán, Ulises Mercado-Salgado, Gladys Alejandra Toledo-Ibarra, Karina Janice Díaz-Reséndiz, and Manuel Iván Girón-Pérez.
• Universidad Autónoma de Nayarit, Laboratorio Nacional de Investigación para la Inocuidad Alimentaria (LANIIA)-Unidad Nayarit, Calle Tres S/N, Colonia. Cd. Industrial, Z.P. 63000, Tepic, México.
• J. Occup. Environ. Med. 2021 Jul 1; 63 (7): 541-547.

ObjectiveTo perform an improved large-scale SARS-CoV-2 detection on pooled tests of asymptomatic workers.MethodsqRT-PCR validation of the SARS-CoV-2 detection in salivae samples and saliva pools and working-group saliva pooling and testing for SARS-CoV-2.ResultsWe found a high Cycle threshold correlation (r = 0.9099) between swabs and saliva samples. Then, through the pooling strategy, we detected that 18/360 (5%) of individual saliva samples were SARS-CoV-2 positive. Saliva-pooling efficiency (360 of test sample/30 individual PCR) was higher (5.45) than the reported for swabbing group-testing and we spared 82% of the PCR reagents as well as sampling and personal protection equipment.ConclusionThrough this simplified and less expensive procedure, we detected in a short time asymptomatic-infected SARS-CoV-2-carriers that were isolated from their co-workers, thus, this methodology can be implemented in different workplaces to ensure consumers that employees are not infectious.Copyright © 2021 American College of Occupational and Environmental Medicine.

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