• Francisco J Manzano-Moreno, Rosa Medina-Huertas, Javier Ramos-Torrecillas, Olga García-Martínez, and Concepción Ruiz.
• Department of Stomatology (Head: Prof. Pilar Aranda-Ramirez), School of Dentistry, University of Granada, Spain; Biomedical Group (BIO277), Department of Nursing (Head: Prof. Pilar Aranda-Ramirez), Faculty of Health Sciences, University of Granada, Spain; Instituto Investigación Biosanitaria, ibs.Granada (Head: Prof. Nicolas Olea-Serrano), Spain.
• J Craniomaxillofac Surg. 2015 Nov 1; 43 (9): 1926-32.

ObjectivesThe objective of this study was to determine the effect of LLDL therapy on the gene expression of osteoblast markers of growth and differentiation.Materials And MethodsThe MG-63 cell line was exposed to diode laser (ezLase) of 940 nm at 1-1.5 W and 3-4 J, and gene expressions (Runx-2, alkaline phosphatase [ALP], type I collagen [Col-I], osterix [OSX], osteocalcin [OSC], osteoprotegerin [OPG], bone morphogenetic protein [BMP]-2 and -7, transforming growth factor-β1 [TGF-β1], and TGF-β receptors [TGF-β R1, TGF-β R2; TGF-β R3]) were evaluated by quantitative RT-PCR.ResultsLLDL treatment stimulated the expression of osteoblast differentiation markers ALP, Col-I, Runx-2, and OSX in relation to the doses applied (P < 0.05), but no changes were detected in OSC, OPG, or BMP-7 at any study dose. This effect may be mediated by TGF-β1 and BMP-2, given that the treatment increased their expression and that of TGF-β receptors R1, R2, and R3 (P < 0.001).ConclusionThese results suggest that the biostimulatory effect of laser therapy on osteoblasts may be attributable to the release of autocrine factors in response to the irradiation. A clinical trial is warranted to test its therapeutic usefulness in bone tissue regeneration and to define a treatment protocol.Copyright © 2015 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

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