• Exp Ther Med · Oct 2017

    Expression of LOX-1 in human mesangial cells is increased by Ox-LDL and IL-1β treatment.

    • Yinghui Deng, Na Lin, Leiyun Wu, Qaing Jia, and Hua Liu.
    • Department of Nephrology, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China.
    • Exp Ther Med. 2017 Oct 1; 14 (4): 3632-3636.

    AbstractThe present study aimed to determine whether the expression of lectin-like oxidized LDL receptor 1 (LOX-1) could be induced by oxidized low-density lipoprotein (Ox-LDL) and interleukin 1β (IL-1β) in human mesangial cells (HMCs). Oil Red O staining was used to observe the uptake of Ox-LDL by HMCs stimulated with IL-1β, and reverse transcription-quantitative polymerase chain reaction analysis and western blotting were used to examine the expression of LOX-1 in HMC following Ox-LDL and IL-1β treatment. Uptake of Ox-LDL by HMCs was upregulated upon stimulation with IL-1β. Furthermore, Ox-LDL (10-40 µg/ml) treatment induced LOX-1 mRNA and protein expression in a dose-dependent manner. In addition, when HMCs were treated with IL-1β and Ox-LDL, the expression of LOX-1 was enhanced further. These results indicated that inhibiting LOX-1 expression or inhibiting the Ox-LDL/LOX-1 signaling axis may be a potential novel method for treating renal disease.

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