• Somayeh Azimi, Kafil Hossein Samadi HS Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Hossein Bannazadeh Baghi, Saeed Shokrian, Khadijeh Najaf, Mohammad Asgharzadeh, Mehdi Yousefi, Firooz Shahrivar, and Mohammad Aghazadeh.
• Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
• GMS Hyg Infect Control. 2016 Jan 1; 11: Doc04.

BackgroundPseudomonas aeruginosa, as Gram-negative rod bacilli, has an important role in human infection. In the present study we aimed to investigate the presence of exo genes and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran.Material And Methods160 isolates of P. aeruginosa were collected and identified by biochemical tests and were characterized for antibiotic resistance. Biofilm production was evaluated by microtiter plate assay and the presence of exo genes was evaluated by allele-specific PCR (polymerase chain reaction). Chi-square test was used for statistical analysis.ResultsThe most effective antibiotics against isolates were colistin and polymyxin B. 87% of the isolates were biofilm producers of which 69% were strongly biofilm producers. 55% of the isolates carried exoY, 52% of the isolates carried exoU, and 26.3% and 5% carried exoS and exoT, respectively.ConclusionOur findings showed different distribution of exo genes in clinical isolates of P. aeruginosa in Northwest Iran. ExoS and exoU were more prevalent in non-biofilm producers and exoY was more prevalent in biofilm producer isolates. These results might indicate the importance of exoY in biofilm production of Pseudomonas aeruginosa.

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