• Injury · Jul 2021

    Punch shear test for quantitative evaluation of bone defect repair in Wistar rat tibia.

    • José Ricardo Lenzi Mariolani, João Fernando Saraiva, Nilza Alzira Batista, and William Dias Belangero.
    • Laboratory of Biomaterials in Orthopaedics, School of Medical Sciences, University of Campinas, Brazil. Electronic address: jrlm@unicamp.br.
    • Injury. 2021 Jul 1; 52 Suppl 3: S13-S17.

    IntroductionBone healing is a complex process influenced by biological and mechanical factors that restores the injured bone's load bearing function. Qualitative methods are usually employed to assess this repair process. This study presents a method of quantitative evaluation of bone repair in circular defects produced in the medial proximal metaphysis of the tibia of rats by means of punch shear tests (PST).ObjectiveTo describe and test a mechanical punch shear test designed to quantify over time the regeneration of bone defects produced in Wistar rats' tibiae.Material And MethodsIn seven experimental groups, 3.2 mm in diameter and 1.5 mm deep defects were drilled on the medial surface of both proximal tibial metaphyses of the animals, just below the joint surfaces. The animals of an additional group were maintained with intact tibiae. After one, two, three, four, six, eight and 12 weeks of follow-up, the animals were sacrificed and the tibiae removed. After removing the soft tissue, the tibial proximal metaphyses were embedded in epoxy resin and cut with a diamond cutting blade, in order to obtain bone slices with equal thickness. The PST were carried out in a universal testing machine and the maximum shear forces were measured.ResultsThe forces increased as a function of the follow-up time in the period of one to three weeks (p <0.001); from this follow-up time on, there were no statistical significant differences between the groups, including the intact tibiae.ConclusionThe test method proved to be suitable to quantitatively assess bone regeneration in monocortical defects produced in the proximal metaphysis of rat tibia within one and three weeks after defect production.Copyright © 2021. Published by Elsevier Ltd.

      Pubmed     Full text   Copy Citation     Plaintext  

      Add institutional full text...

    Notes

     
    Knowledge, pearl, summary or comment to share?
    300 characters remaining
    help        
    You can also include formatting, links, images and footnotes in your notes
    • Simple formatting can be added to notes, such as *italics*, _underline_ or **bold**.
    • Superscript can be denoted by <sup>text</sup> and subscript <sub>text</sub>.
    • Numbered or bulleted lists can be created using either numbered lines 1. 2. 3., hyphens - or asterisks *.
    • Links can be included with: [my link to pubmed](http://pubmed.com)
    • Images can be included with: ![alt text](https://bestmedicaljournal.com/study_graph.jpg "Image Title Text")
    • For footnotes use [^1](This is a footnote.) inline.
    • Or use an inline reference [^1] to refer to a longer footnote elseweher in the document [^1]: This is a long footnote..

    hide…