• Shu-Yang Li, Yoichi Yoshida, Eiichi Kobayashi, Akihiko Adachi, Seiichiro Hirono, Tomoo Matsutani, Seiichiro Mine, Toshio Machida, Mikiko Ohno, Eiichiro Nishi, Yoshiro Maezawa, Minoru Takemoto, Koutaro Yokote, Kenichiro Kitamura, Makoto Sumazaki, Masaaki Ito, Hideaki Shimada, Hirotaka Takizawa, Koichi Kashiwado, Go Tomiyoshi, Natsuko Shinmen, Rika Nakamura, Hideyuki Kuroda, Xiao-Meng Zhang, Hao Wang, Kenichiro Goto, Yasuo Iwadate, and Takaki Hiwasa.
• Department of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba 260‑8670, Japan.
• Int. J. Mol. Med. 2020 Oct 1; 46 (4): 1274-1288.

AbstractThe aim of the present study was to identify novel antibody markers for the early diagnosis of atherosclerosis in order to improve the prognosis of patients at risk for acute ischemic stroke (AIS) and acute myocardial infarction (AMI). A first screening involved the serological identification of antigens by recombinant cDNA expression cloning and identified additional sex combs‑like 2 (ASXL2) as a target antigen recognized by serum IgG antibodies in the sera of patients with atherosclerosis. Antigens, including the recombinant glutathione S‑transferase‑fused ASXL2 protein and its synthetic peptide were then prepared to examine serum antibody levels. Amplified luminescence proximity homogeneous assay‑linked immunosorbent assay, which incorporates glutathione‑donor beads and anti‑human‑IgG‑acceptor beads, revealed significantly higher serum antibody levels against the ASXL2 protein and its peptide in the patients with AIS, diabetes mellitus, AMI, chronic kidney disease, esophageal squamous cell carcinoma, or colorectal carcinoma compared with those in healthy donors. The ASXL2 antibody levels were well associated with hypertension complication, but not with sex, body mass index, habitual smoking, or alcohol intake. These results suggest that the serum ASXL2 antibody marker can discriminate between hypertension‑induced atherosclerotic AIS and AMI, as well as a number of digestive organ cancers.

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