• Jôse Mára de Brito, Mariângela Macchione, Kelly Yoshizaki, Alessandra Choqueta Toledo-Arruda, Beatriz Mangueira Saraiva-Romanholo, Maria de Fátima Andrade, Thaís Mauad, Dolores Helena Rodriguez Ferreira Rivero, and Saldiva Paulo Hilário Nascimento PH Department of Pathology, Laboratory of Experimental Air Pollution, School of Medicine, University of São Paulo, São Paulo, Brazil;.
• Department of Pathology, Laboratory of Experimental Air Pollution, School of Medicine, University of São Paulo, São Paulo, Brazil; josemarabrito@usp.br.
• J. Appl. Physiol. 2014 Sep 1; 117 (5): 492-9.

AbstractAmbient particles may undergo modifications to their chemical composition as a consequence of climatic variability. The determination of whether these changes modify the toxicity of the particles is important for the understanding of the health effects associated with particle exposure. The objectives were to determine whether low levels of particles promote cardiopulmonary effects, and to assess if the observed alterations are influenced by season. Mice were exposed to 200 μg/m(3) concentrated ambient particles (CAPs) and filtered air (FA) in cold/dry and warm/humid periods. Lung hyperresponsiveness, heart rate, heart rate variability, and blood pressure were evaluated 30 min after each exposure. After 24 h, blood and tissue samples were collected. During both periods (warm/humid and cold/dry), CAPs induced alterations in red blood cells and lung inflammation. During the cold/dry period, CAPs reduced the mean corpuscular volume levels and increased erythrocytes, hemoglobin, mean corpuscular hemoglobin concentration, and red cell distribution width coefficient variation levels compared with the FA group. Similarly, CAPs during the warm/humid period decreased mean corpuscular volume levels and increased erythrocytes, hemoglobin, hematocrit, and red cell distribution width coefficient variation levels compared with the FA group. CAPs during the cold/dry period increased the influx of neutrophils in the alveolar parenchyma. Short-term exposure to low concentrations of CAPs elicited modest but significant pulmonary inflammation and, to a lesser extent, changes in blood parameters. In addition, our data support the concept that changes in climate conditions slightly modify particle toxicity because equivalent doses of CAPs in the cold/dry period produced a more exacerbated response. Copyright © 2014 the American Physiological Society.

23 keywords...

hide…