• Marcelo C da Costa, Bruno A Ferreira, Francyelle B R de Moura, Lara G de Lima, Fernanda de A Araujo, and Francisco C D Mota.
• Department of Veterinary Surgery, Paulista State University Júlio de Mesquita Filho, Access Road Highway Professor Paulo Donato Castelane Castelane S / N, Vila Industrial, [Zip Code]: 14884-900, Jaboticabal-SP, Brazil. Electronic address: marcelocarrijo@hotmail.com.
• Injury. 2021 Aug 1; 52 (8): 2075-2083.

AbstractWounds are conditions largely present in the clinical routine, and even though frequent, their complete resolution can be challenging. Several solutions can aid or stimulate the healing process, and for this reason, this work used a stabilized solution of 4% sodium hypochlorite for the treatment of excisional wounds in mice. This study was carried out in two distinct stages: in the first stage, the optimal concentration of the chlorinated solution was determined by using the sponge implantation technique in mouse subcutaneous tissue to evaluate the dose-response curve; and in the second phase, this concentration was tested in an experimental model of excisional skin wounds in mice. Soluble collagen, hemoglobin, myeloperoxidase (MPO) and N-acetyl-β-D-glycosaminidase (NAG) activity were assessed, and total, type I and type III collagen deposition were quantified in both stages. Based on the results presented in the sponge implantation study, the chlorinated solution at 150 ppm (0.015%) was chosen for use in a preclinical trial of skin healing in mice. At 1, 3, 7 and 14 days of treatment, the % wound area repair in the group treated with 150 ppm chlorinated solution was higher when compared to the control group, with statistical differences at all time points (*p≤ 0.05 and **p≤ 0.01). 150 ppm chlorinated solution obtained from a stabilized 4% sodium hypochlorite solution was effective in accelerating cutaneous excision wound repair in mice, showing a positive influence on tissue repair.Copyright © 2021. Published by Elsevier Ltd.

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