• A Atik, A S Avcikurt, and S Sargin.
• Department of Orthopaedics and Traumatology, Faculty of Medicine, Balikesir, Turkey.
• Niger J Clin Pract. 2021 May 1; 24 (5): 647-650.

BackgroundHow the meniscus adapts to the morphological changes in the lateral tibiofemoral compartment, in terms of gene expression, was the reason to establish this present study.ObjectiveThis study aimed to determine the changes in the mRNA levels of the apoptotic and autophagic genes in the discoid meniscus.Subjects And MethodsWe have investigated the apoptotic and autophagic gene levels in discoid and normal lateral menisci of 21 patients (11 discoid and 10 control). The RNAs were isolated from the fresh discoid and healthy meniscal tissue. Gene expression was defined based on the threshold cycle (Ct), and Actin beta was used as a reference gene that acts as an internal reference to normalize RNA expression, which was calculated as 2-ΔΔCT.ResultsApoptotic and autophagic gene levels were significantly higher in the discoid meniscus group. In discoid meniscus samples, the Bcl-2 mRNA, BclXL, BAK mRNA, ATG 12, ATG 7, ATG 5, ATG 3, and Beclin1 mRNA levels were higher by 4.2, 5.9, 9.1, 8.3, 23.2, 6.1, 12.4, and 18.1 times, respectively, with statistically significant differences (p < 0.001).ConclusionThe discoid meniscus etiology should be considered both in morphological and genetic modulation manners: apoptotic and autophagic genes play roles with tibiofemoral morphological differences.

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