• Bo Yang, Rong Wang, Lin-Lin Ji, Xiao-Ping Li, Xiao-He Li, Hong-Gang Zhou, Zhan-Kun He, Hong-Liang Xu, Fan-Jie Meng, and Guang-Shun Wang.
• Department of Thoracic Surgery, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin, P.R. China.
• Shock. 2022 Feb 1; 57 (2): 212220212-220.

ObjectiveGinsenoside Rd (GSRd) displays a variety of pharmacological effects. However, the underlying role in acute lung injury (ALI) is not clear. In this study, the protective effect of GSRd on lipopolysaccharide (LPS)-induced ALI is investigated to explore the potential mechanisms.MethodsGSRd-target-ALI-related gene set was constructed. And bioinformatics tools were used to discover the potential mechanism. We observed the survival of subjects for 72 h. In addition, male BALB/c mice were intraperitoneal injected with GSRd (25 and 50 mg/kg) after received one intratracheal instillation of LPS. Inflammatory changes, oxidative stress, and phosphorylation were assessed to study the biological effects.ResultsA total of 245 interaction genes were collected. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were enriched in immune-inflammatory system. Among them, PI3K-Akt signaling pathway was the highest-ranked pathway of inflammatory response. In vivo study, it was found that GSRd improved survival in endotoxemic mice and inhibited the major characteristic of ALI. And the p-PI3K and p-Akt expression was significantly decreased by GSRd treatment.ConclusionGSRd could protect mice against LPS-induced ALI effectively by inhibiting the PI3K-Akt signaling pathway.Copyright © 2021 by the Shock Society.

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