• Lisa J Shi, Xiwei Tang, Jiang He, and Weibin Shi.
• Department of Radiology and Medical Imaging, University of Virginia, Charlottesville, VA, USA.
• Am. J. Med. Sci. 2021 Sep 1; 362 (3): 297-302.

BackgroundGlucometers are widely used in animal research due to simplicity and ease of utilization, but their accuracy in blood glucose assessment for hyperlipidemic mice is unknown.MethodsHere, we compared blood glucose levels measured by a glucometer with plasma glucose levels measured by a standard enzymatic assay for 325 genetically diverse F2 mice derived from LP and BALB/c (BALB) Apoe-/- mice. Non-fasting glucose levels were measured before initiation of a Western diet and after 11 weeks on the diet.ResultsOn chow diet, lab-measured plasma glucose levels were 279.5 ± 42.6 mg/dl (mean ± SD), while blood glucose values measured by glucometer were 138.7 ± 16.6 mg/dl. The two measures had no correlation (R2 = 0.006, p = 0.167). On the Western diet, plasma glucose levels rose to 351.1 ± 121.6 mg/dl, while glucometer-measured blood glucose fell to 128.7 ± 27.9 mg/dl. The two measures showed a moderate correlation (R2 = 0.111, p = 3.1E-9). Lab-measured plasma glucose showed strong positive correlations with plasma triglyceride and non-high-density lipoprotein cholesterol levels, while glucometer-measured blood glucose showed an inverse correlation with non-high-density lipoprotein levels on the chow diet.ConclusionsOur results indicate that hyperlipidemia affects the accuracy of glucometers in measuring blood glucose levels of mice.Copyright © 2021 Southern Society for Clinical Investigation. Published by Elsevier Inc. All rights reserved.

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