• Antonija Babić, Lejla Kurić, Ana Zelić Kerep, Lana Desnica, Antonela Lelas, Milan Milošević, Ranka Serventi-Seiwerth, Nadira Duraković, Zinaida Peric, Marinka Mravak Stipetić, Ervina Bilic, Romana Čeović, Marko Barešić, Tamara Vukić, Dina Ljubas Kelečić, Sanja Mazić, Ines Bojanić, Ana Hećimović, Ernest Bilic, Renata Zadro, Radovan Vrhovac, Steven Z Pavletic, Drago Batinić, and Dražen Pulanić.
• Antonija Babić, Department of Laboratory Diagnostics, University Hospital Centre Zagreb, Kišpatićeva 12, 10000 Zagreb, ababic2@kbc-zagreb.hr.
• Croat. Med. J. 2021 Apr 30; 62 (2): 154-164.

AimTo assess the correlations of B regulatory cells (Bregs) and monocyte subsets in peripheral blood with the National Institutes of Health (NIH)-consensus-defined clinical manifestations of chronic graft-vs-host disease (cGvHD), in an attempt to establish their role as cellular biomarkers.MethodsThis multidisciplinary prospective study enrolled adult cGVHD patients treated in the University Hospital Center Zagreb and University of Zagreb School of Medicine. Immunophenotypic subpopulations of CD24highCD38high Bregs (CD27-, CD27+, and total) and monocyte (classical, intermediate, and non-classical) counts were correlated with demographic, transplant, and cGVHD-related data. Bivariate correlation analysis was performed to evaluate the correlations between Bregs and monocytes subsets and cGVHD organ involvement, as well as cGVHD severity and immunosuppression intensity.ResultsTwenty-two adult patients (54.5% female) with cGVHD were enrolled. The median (range) age was 44.5 years (24-65). All patients were transplanted for hematologic malignancies and 40.9% had severe NIH cGVHD global score. The median time from cGVHD diagnosis to the analysis was 16.6 months (0-176). The organ most frequently affected with cGVHD were the eyes (68.2%), skin (45.5%), lungs (45.5%), and liver (40.9%). Lower total and CD27-Bregs counts were correlated with worse cGVHD severity, higher immunosuppression intensity, and lung cGVHD, in terms of cell count, but also with skin cGVHD, in terms of percentages. Patients with liver and joint/fascia cGVHD had a lower percentage of non-classical monocytes and patients with more severe global NIH score had a higher classical monocytes count.ConclusionDifferent organs affected by cGVHD are differently associated with different subpopulations of Bregs and monocytes.

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