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Zhonghua Bing Li Xue Za Zhi · Dec 2006
[Study of loss of heterozygosity in oligodendroglial tumors by real-time quantitative polymerase chain reaction-based microsatellite analysis].
- Shu-kun Zhang, De-hong Lu, Yue-shan Piao, Yan-ning Cai, and Qing-zhong Xu.
- Department of Pathology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
- Zhonghua Bing Li Xue Za Zhi. 2006 Dec 1; 35 (12): 731-4.
ObjectiveTo study the loss of heterozygosity (LOH) at chromosomes 1p or 19q in oligodendroglial tumors.MethodsTwenty-eight cases of oligodendroglial tumors were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on paraffin-embedded tumor tissues in order to study the status of chromosomes 1p and 19q.ResultsAmong the 28 cases of oligodendroglial tumors, 24 cases (85.7%) showed 1p LOH, while 18 cases (64.3%) showed 19q LOH and 17 cases (60.7%) showed LOH of both 1p and 19q. LOH at 1p or 19q was present in 25 (89.3%) of the 28 cases.ConclusionsReal-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific way in detecting LOH in paraffin-embedded tumor tissues. LOH at 1p or 19q is present in majority of the oligodendroglial tumors studied.
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