• Adi Muradi Muhar, Agung Putra, Vito Mahendra Ekasaputra, DarlanDewi MasyithahDMDepartment of Parasitology, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia., Desiree Anggia Paramita, and Adeputra NasutionIqbal PahleviIPDepartment of Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia..
• Department of Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia.
• Med Glas (Zenica). 2021 Aug 1; 18 (2): 398-403.

AbstractAim To investigate the role of hypoxia-preconditioned mesenchymal stem cells (H-MSCs) in preventing peritoneal adhesion by regulating IL-6 at days 6 and 12. Methods Twenty-four PAs rat model weighing 250 g to 300 g were randomly allocated into 4 groups: sham (Sh), control (C), H-MSCs treatment group at dose 1.5 x 106(T1) and 3 x 106(T2). To induce H-MSCs, all MSCs population were incubated under hypoxia state (5% O2 ), 5% CO2, and 37oC for 24 hours. Expression level of IL-6 was performed using ELISA. Morphological appearance of adhesion was observed by visualizing the existence of adhesion formation in intestinal. Results In this study we found that there was a trend of decrease of IL-6 level on day 6 following MSCs treatments. Interestingly, there was a significant decrease of IL-6 level on day 12 in all treatment groups. Also, no adhesion occurred in T2 group. Conclusions H-MSCs prevent PA development by suppressing the prolonged release of IL-6 at proliferation phase.Copyright© by the Medical Assotiation of Zenica-Doboj Canton.

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