• J Ethnopharmacol · Mar 2011

    Panax notoginseng saponins promotes proliferation and osteogenic differentiation of rat bone marrow stromal cells.

    • Xue-Dong Li, Jian-Sheng Wang, Bo Chang, Bin Chen, Chun Guo, Guo-Qing Hou, Dong-Yang Huang, and Shi-Xin Du.
    • Department of Orthopaedics, The First Affiliated Hospital, Shantou University Medical College, 57 Chang Ping Road, Shantou, Guangdong 515041, China.
    • J Ethnopharmacol. 2011 Mar 24; 134 (2): 268-74.

    Aim Of The StudyPanax notoginseng saponins (PNS) is the main effective component of Panax notoginseng, have various pharmacologic activities such as antioxidant, anti-inflammatory, and estrogen-like bioactivities, have been shown to be an effective agent on anti-osteoporosis. Bone marrow stromal cells (BMSCs) play a crucial homeostatic role in skeletal modeling and remodeling due to their capability to differentiate into osteooblasts. Whether PNS has effect on osteogenic differentiation of BMSCs are unknown. This study was designed to investigate the effects of PNS on the proliferation and osteogenic differentiation of BMSCs in vitro.Materials And MethodsWhen BMSCs cultivated in the basal medium or the osteogenic induction medium (OS with or without PNS), cell proliferation was analyzed using an MTT assay, the mineralization was assessed using Alizarin red S staining, the alkaline phosphatase activity was measured using a commercial kit, the mRNA level of osteogenic gene and PPARγ2 gene were determined using RT-PCR, the protein level of PPARγ2 was analyzed by Western blotting.ResultsBMSCs cultured in the basal medium with PNS caused a significant increase in proliferation. PNS treatment increased ALP activity, Alizarin red S staining and mRNA level of ALP, Cbfa 1, OC, and BSP, whereas decreased the mRNA level and protein expression of PPARγ2 during osteogenic induction. In addition, the effects of PNS treatment were dose-dependent relationship.ConclusionPNS could stimulate BMSCs proliferation and promote their osteogenic differentiation by up-regulation expression of osteogenic marker gene and down-regulation expression of adipogenic marker gene in a dose-dependent manner. Thus, PNS may play an important therapeutic role in osteoporosis patients by improving osteogenic differentiation of BMSCs.Crown Copyright © 2010. Published by Elsevier Ireland Ltd. All rights reserved.

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