• Kun Li, Feng Li, Li-Juan Guo, Jie Li, Ke Huang, Wei-Hua Guo, and Wei-Dong Tian.
• State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
• Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 May 1; 44 (3): 337-41.

ObjectiveTo investigate the impact of combined adipose-derived stem cells (ASCs) and platelet-rich plasma (PRP) on the survival of transplanted fat.MethodsThe ASCs were isolated and cultured from fat tissues by enzyme digestion; and the PRP was prepared by two-step ultracentrifugation. The grafts of fat granules were divided into test groups (ASCs + PRP + fat granules, PRP + fat granules, ASCs + fat granules) and control groups (PBS + fat granules). The grafts were injected into the left and right dorsal subcutaneous areas of nude mice. General observations, volume measurements and microscope examinations were conducted 10 d, 30 d, 60 d and 90 d after transplantation.ResultsThe grafts of the mice in the ASCs + PRP group showed soft structure, with light yellow color and closer to normal adipose tissue compared with those in other groups. Greater survival volumes (P < 0.05) and better histology were also observed in the grafts of the mice in the ASCs + PRP group.ConclusionThe fat grafts consisting of PRP and ASCs constitute an ideal transplant strategy, which could provide a valuable and needed tool in plastic and reconstructive surgery.

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