• Clinics · Jul 2016

    Effects of ganglioside G(M1) and erythropoietin on spinal cord lesions in rats: functional and histological evaluations.

    • Raphael Martus Marcon, Alexandre Fogaça Cristante, Tarcísio Eloy Pessoa de Barros, Ricardo Ferreira, and Dos SantosGustavo BispoGBInstituto de Ortopedia e Traumatologia, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (IOT-HCFMUSP), Divisão de Cirurgia de Coluna Vertebral, Laboratório de Investigação Médica (LIM 41), São Paulo/SP,.
    • Instituto de Ortopedia e Traumatologia, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (IOT-HCFMUSP), Divisão de Cirurgia de Coluna Vertebral, Laboratório de Investigação Médica (LIM 41), São Paulo/SP, Brazil.
    • Clinics (Sao Paulo). 2016 Jul 1; 71 (6): 351-60.

    ObjectiveTo evaluate the functional and histological effects of ganglioside G(M1) and erythropoietin after experimental spinal cord contusion injury.MethodsFifty male Wistar rats underwent experimental spinal cord lesioning using an NYU-Impactor device and were randomly divided into the following groups, which received treatment intraperitoneally. The G(M1) group received ganglioside G(M1) (30 mg/kg); the erythropoietin group received erythropoietin (1000 IU/kg); the combined group received both drugs; and the saline group received saline (0.9%) as a control. A fifth group was the laminectomy group, in which the animals were subjected to laminectomy alone, without spinal lesioning or treatment. The animals were evaluated according to the Basso, Beattie and Bresnahan (BBB) scale, motor evoked potential recordings and, after euthanasia, histological analysis of spinal cord tissue.ResultsThe erythropoietin group had higher BBB scores than the G(M1) group. The combined group had the highest BBB scores, and the saline group had the lowest BBB scores. No significant difference in latency was observed between the three groups that underwent spinal cord lesioning and intervention. However, the combined group showed a significantly higher signal amplitude than the other treatment groups or the saline group (p<0.01). Histological tissue analysis showed no significant difference between the groups. Axonal index was significantly enhanced in the combined group than any other intervention (p<0.01).ConclusionG(M1) and erythropoietin exert therapeutic effects on axonal regeneration and electrophysiological and motor functions in rats subjected to experimental spinal cord lesioning and administering these two substances in combination potentiates their effects.

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