• Atsunori Nakao, Kei Kimizuka, Donna B Stolz, Seda NetoJoaoJ, Takashi Kaizu, Augustine M K Choi, Takashi Uchiyama, Brian S Zuckerbraun, Anthony J Bauer, Michael A Nalesnik, Leo E Otterbein, David A Geller, and Noriko Murase.
• University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA.
• Surgery. 2003 Aug 1; 134 (2): 285-92.

BackgroundHeme oxygenase (HO)-1 system has been shown to provide protection against oxidative stress through the degradation of heme to biliverdin, free iron, and carbon monoxide (CO). This study investigated cytoprotective efficacy of CO at a low concentration on cold ischemia/reperfusion (I/R) injury of transplanted intestine.MethodsLewis rat recipients of syngenic orthotopic small intestinal transplantation with 6 hours UW cold preservation were either kept in room air (air-treated control) or exposed to CO (250 ppm) for 1 hour before and 24 hours after surgery.ResultsIn air-treated grafts, mRNA levels for interleukin-6, intracellular adhesion molecule-1, cyclooxygenase-2, and inducible nitric oxide synthase promptly increased. Sequential histopathologic analysis of untreated grafts revealed initial rapid epithelial loss, subsequent recruitment of inflammatory infiltrates, and local hemorrhage in the lamina propria, which extended downward to the epithelial crypt and muscle layer with time. CO effectively blocked proinflammatory cascade during I/R injury, inhibited upregulation of inflammatory molecules and ameliorated intestinal tissue injuries. Beneficial effects of CO were associated with improved graft blood flow without inhibiting endogenous HO-1 activity. Recipient animal survival was significantly improved with CO to 100% versus 58% in air-treated controls.ConclusionsThese results indicate a significant role for CO in protecting the intestine from cold I/R injury associating with small intestinal transplantation.

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