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Zhongguo Zhong Yao Za Zhi · May 2015
[Simultaneous determination of seven components in Pudilan Xiaoyan oral liquid by HPLC].
- Zi-bo Dong, Chao Li, and Jian-guo Shao.
- Zhongguo Zhong Yao Za Zhi. 2015 May 1; 40 (9): 1747-50.
ObjectiveTo establish an HPLC method for the content determination of baicalin, wogonin, chlorogenic acid, caffeic acid, cichoric acid, corynoline and adenosine in Pudilan Xiaoyan oral liquid.MethodThe analysis was performed on a Phenomenex Luna C18 column (4.6 mm x 250 mm, 5 µm) with a gradient mobile phase of methanol-0.1% trifluoroacetic acid solution system at flow rate of 1.0 mL · min(-1). The detective wavelength was at 280 nm. The column temperature was 30 °C.ResultThe standard curves of seven studied components show good linearity in their concentration ranges with r ≥ 0.999 6. The average recovery was 98.73%-102.1% with RSD less than 2.6%.ConclusionThe method is rapid, simple and accurate, and can be applied for the quality control of Pudilan Xiaoyan oral liquid.
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