• Nanoscale · Jun 2013

    Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins.

    • Rabeka Alam, Joshua Zylstra, Danielle M Fontaine, Bruce R Branchini, and Mathew M Maye.
    • Department of Chemistry, Syracuse University, Syracuse, New York 13244, USA.
    • Nanoscale. 2013 Jun 21; 5 (12): 5303-6.

    AbstractSequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.

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