• BioTechniques · Jun 2010

    Bioluminescence imaging of dual gene expression at the single-cell level.

    • Hyuckjoon Kwon, Toshiteru Enomoto, Masahiro Shimogawara, Kazunori Yasuda, Yoshihiro Nakajima, and Yoshihiro Ohmiya.
    • Regenerative Medicine/Tissue Engineering Division, Research Center for Cooperative Projects, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
    • BioTechniques. 2010 Jun 1; 48 (6): 460-2.

    AbstractBioluminescence imaging reveals the long-term dynamics of individual gene expression in a single cell. However, methods for simultaneously imaging multiple gene expression patterns have been unknown to date. Here, we constructed a dual-path optical luminescence imaging system using a two-color reporter system and could simultaneously track two gene expression patterns for several days in a single cell.

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