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Review
Tandem Mass Spectrum Sequencing: An Alternative to Database Search Engines in Shotgun Proteomics.
- Thilo Muth, Erdmann Rapp, Frode S Berven, Harald Barsnes, and Marc Vaudel.
- Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
- Adv Exp Med Biol. 2016 Jan 1; 919: 217-226.
AbstractProtein identification via database searches has become the gold standard in mass spectrometry based shotgun proteomics. However, as the quality of tandem mass spectra improves, direct mass spectrum sequencing gains interest as a database-independent alternative. In this chapter, the general principle of this so-called de novo sequencing is introduced along with pitfalls and challenges of the technique. The main tools available are presented with a focus on user friendly open source software which can be directly applied in everyday proteomic workflows.
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