• Cancer letters · Nov 2013

    Review

    Analyzing the cancer methylome through targeted bisulfite sequencing.

    • Eun-Joon Lee, Junfeng Luo, James M Wilson, and Huidong Shi.
    • GHSU Cancer Center, Department of Biochemistry and Molecular Biology, Georgia Health Sciences University, Augusta, GA 30912, USA.
    • Cancer Lett. 2013 Nov 1; 340 (2): 171-8.

    AbstractBisulfite conversion of genomic DNA combined with next-generation sequencing (NGS) has become a very effective approach for mapping the whole-genome and sub-genome wide DNA methylation landscapes. However, whole methylome shotgun bisulfite sequencing is still expensive and not suitable for analyzing large numbers of human cancer specimens. Recent advances in the development of targeted bisulfite sequencing approaches offer several attractive alternatives. The characteristics and applications of these methods are discussed in this review article. In addition, the bioinformatic tools that can be used for sequence capture probe design as well as downstream sequence analyses are also addressed. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

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