• Ingrid Carruyo, Yusmary Fernández, Letty Marcano, Xiomara Montiel, and Zaida Torrealba.
• Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela. yncarr24@hotmail.com
• Biol Trace Elem Res. 2008 Dec 1; 125 (3): 276-85.

AbstractThe present study determines lead content in onion root tip cells (Allium cepa L.), correlating it with its toxicity. The treatment was carried at 25 +/- 0.5 degrees C using aqueous solutions of lead chloride at 0.1, 0.25, 0.50, 0.75, and 1 ppm for 12, 24, 48, and 72 h. For each treatment, a control where the lead solution was substituted by distilled water was included. After treatment, the meristems were fixed with a mixture of alcohol-acetic acid (3:1) and colored according to the technique of Feulgen. Lead content was quantified by graphite furnace absorption atomic spectrometry. The lead content in the roots ranged from 3.25 to 244.72 microg/g dry weight, with a direct relation with the concentration and time of exposure. A significant negative correlation was presented (r = -0.3629; p < 0.01) among lead content and root growth increment, and a positive correlation (r = 0.7750; p < 0.01) with the induction of chromosomic aberrations. In conclusion, lead is able to induce a toxic effect in the exposed roots, correlated with its content.

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