• Radiother Oncol · Mar 2008

    Comparative Study

    Biological effect of intermittent radiation exposure in vivo: recovery from sublethal damage versus reoxygenation.

    • Natsuo Tomita, Yuta Shibamoto, Masato Ito, Hiroyuki Ogino, Chikao Sugie, Shiho Ayakawa, and Hiromitsu Iwata.
    • Department of Radiology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. ntomita@med.nagoya-cu.ac.jp
    • Radiother Oncol. 2008 Mar 1; 86 (3): 369-74.

    PurposeIn vivo effects of intermittent irradiation are influenced by recovery from sublethal damage (SLDR) and reoxygenation, so contribution of the two factors were investigated using murine tumors.Methods And Materials1-cm-diameter SCCVII tumors growing in the legs of C3H/HeN mice were used. First, effects of 5 fractions of 6 Gy given at intervals of 2.5-15 min were compared using an in vivo-in vitro assay, by clamping the tumor-bearing legs to exclude the influence of reoxygenation. In the second and third experiments, changes in the hypoxic fraction at 0-15 min after 13 or 5 Gy were assessed by a paired cell survival method. Fourth, effects of 5 fractions of 5 Gy given at intervals of 3-10 min under conditions of limited reoxygenation were compared using a growth delay assay.ResultsCell survival from clamped tumors tended to increase with elongation of the intervals, but not significantly. The hypoxic fraction tended to decrease at 5-15 min from the level immediately after irradiation. Effects on tumor growth tended to decrease with elongation of the intervals.ConclusionsReoxygenation occurring within 5-15 min appeared to compensate for SLDR in SCCVII tumors. When reoxygenation was limited, the decrease of radiation effect occurred due to SLDR.

      Pubmed     Full text   Copy Citation  

      Add institutional full text...

    Notes

    hide…

What will the 'Medical Journal of You' look like?

Start your free 21 day trial now.

We guarantee your privacy. Your email address will not be shared.