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- Alexander S Tanas, Marina E Borisova, Ekaterina B Kuznetsova, Viktoria V Rudenko, Kristina O Karandasheva, Marina V Nemtsova, Vera L Izhevskaya, Olga A Simonova, Sergey S Larin, Dmitry V Zaletaev, and Vladimir V Strelnikov.
- Epigenetics Laboratory, Research Centre for Medical Genetics, Moskvorechie Street 1, Moscow 115478, Russia.
- Epigenomics. 2017 Jun 1; 9 (6): 833-847.
AimTo develop a reduced representation bisulfite sequencing (RRBS) approach for rapid and affordable genome-wide DNA methylation analysis.MethodsWe have selected restriction endonuclease XmaI to produce RRBS library fragments. After digestion and partial fill-in DNA fragments were ligated to barcoded adapters, bisulfite converted, size-selected, and sequenced on the Ion Torrent Personal Genome Machine. XmaI-RRBS results were compared with the previously published RRBS data.ResultsWe have developed an XmaI-RRBS method for rapid and affordable genome-wide DNA methylation analysis, with library preparation taking only 4 days and sequencing possible within 4 h. We have also addressed several challenges in order to further improve the RRBS technology. XmaI-RRBS may be performed on degraded DNA samples and is compatible with the bench-top next-generation sequencing machines.
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